Pollen Analysis Slides
Making microscopy slides with honey:
Centrifuge tubes filled with diluted honey (10g to 100ml)
Centrifuge each centrifuge is @2500rpm for 15 minutes
Sediment after processing 10g of honey
PC Blueberry Honey
Here is a PC Blueberry Honey from Lac St-Jean Quebec. The upper image is at x100 magnification. You will also notice that what is branded as Blueberry has very few blueberry pollen grains. It is a smorgasbord of pollen varieties, including some rust and misc fungal spores. This is another objective of the project, to get a better understanding of where are honeys are coming from. Just because we see a dominant flower, i.e. the colonies are sitting in a field of blueberries doesn't mean the honey is exclusively blueberry. Another concept we will look at is over and under representation of pollen types. Fireweeds and Ericaceae spp (blueberry, lingonberry) are well known to be under represented. 0.3 to 50 other pollen grains for Fireweed and 1 to 5 for those in the Ericaceae spp. vs other average types. Luckily most Yukon honeys are much more simple than this one.
The lower image shows calibrated measurements using the microscope's digital camera software. This image is at x400 magnification
Note: I am testing my preparation procedures on these commercial brands ( I have plenty to spare). I will need to add more dye in my mounting agent. This one in particular seems to have very high colloids. I may have to add a 10% KOH solution into my procedures to help dissolve some of this substance.
Pollen Guide for Eastern Canada: